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. 2021 Mar 10;13(6):8797–8816. doi: 10.18632/aging.202688

Figure 2.

Figure 2

Validation of the DEcircRNAs and DElncRNAs. The levels of six DEcircRNAs and eight DElncRNAs were validated in prefrontal cortex samples from Nrf2 (-/-) and Nrf2 (+/+) mice using qRT-PCR and normalized to the internal reference gene (β-actin). (A) The qRT-PCR results for five of the verified circRNAs (all but mmu_circ_0003404) were consistent with the microarray results. (B) The qRT-PCR results for all eight verified lncRNAs were consistent with the microarray results. All experiments were replicated three times. The deep red column indicates the FCs of the DEcircRNAs/DElncRNAs determined through microarray analyses; the blue column indicates the expression FCs of the DEcircRNAs/DElncRNAs determined through qRT-PCR experiments. The presented values are the means ± standard deviations. Values > 1 indicate up-regulated DEcircRNAs/DElncRNAs, and values < 1 indicate down-regulated DEcircRNAs/DElncRNAs. *p < 0.05, **p < 0.01.