Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293T cells | ATCC | CRL3216 | |
| Antibody | α-Tubulin (mouse monoclonal) |
Sigma | T6074 | Immunoblotting dilution (1: 2500) |
| Antibody | RFP (rabbit polyclonal) |
Rockland | 600-401-379 | Immunoblotting dilution (1: 1000) |
| Antibody | GFP (mouse monoclonal) |
Clontech/Takara Bio | Cat# 632380 | Immunoblotting dilution (1: 1000) |
| Antibody | Hemagglutinin (HA) tag (clone 12CA5) (mouse monoclonal) | Roche | Cat# 11583816001 | Immunoblotting dilution (1: 1000) |
| Antibody | MYC tag (9B11) (mouse monoclonal) | Cell Signaling | Cat# 2276 | Immunoblotting dilution (1: 1000) |
| Antibody | Gαi3 (rabbit polyclonal) |
Santa Cruz Biotechnology | Cat# sc-262 | Immunoblotting dilution (1:250) |
| Antibody | Pan-Gβ (rabbit polyclonal) | Santa Cruz Biotechnology | Cat# sc-261 | Immunoblotting dilution (1: 250) |
| Antibody | Goat anti-rabbit Alexa Fluor 680 (goat polyclonal) | Life Technologies | Cat# A21077 | Immunoblotting dilution (1:10,000) |
| Antibody | Goat anti-mouse IRDye 800 (goat polyclonal) | LiCor | Cat# 926–32210 | Immunoblotting dilution (1:10,000) |
| Recombinant DNA reagent | pmRFP-FKBP-pseudojanin (plasmid) |
Addgene | Cat# 37999 | |
| Recombinant DNA reagent | pmRFP-FKBP-GIV GBA (plasmid) |
Parag-Sharma et al., 2016 | Contains human GIV aa1660-1705 | |
| Recombinant DNA reagent | pmRFP-FKBP-AGS1* (plasmid) |
This paper | Contains rat AGS1 with C278S mutation. See details in ‘Plasmids’ section of 'Materials and methods' |
|
| Recombinant DNA reagent | pmRFP-FKBP-Ric-8A* (plasmid) |
This paper | Contains rat Ric-8A aa12-492 See details in ‘Plasmids’ section of 'Materials and methods' |
|
| Recombinant DNA reagent | pmRFP-FKBP-R12 GL (plasmid) |
Maziarz et al., 2020 | Contains mouse RGS12 aa1185-1221 See details in ‘Plasmids’ section of 'Materials and methods' |
|
| Recombinant DNA reagent | Lyn11-FRB (plasmid) |
Parag-Sharma et al., 2016 | ||
| Recombinant DNA reagent | pcDNA3.1-Venus(155-239)-Gβ1
(plasmid) |
Hollins et al., 2009 | For the mammalian expression of Gβ1 tagged with a fragment of Venus (VC-Gβ1). Provided by N. Lambert (Augusta University, Augusta, GA) | |
| Recombinant DNA reagent | pcDNA3.1-Venus(1-155)-Gγ2
(plasmid) |
Hollins et al., 2009 | For the mammalian expression of Gγ2 tagged with a fragment of Venus (VN-Gγ2). Provided by N. Lambert (Augusta University, Augusta, GA) | |
| Recombinant DNA reagent | pcDNA3.1-Gβ1
(plasmid) |
Hollins et al., 2009 | For the mammalian expression of untagged Gβ1. Provided by N. Lambert (Augusta University, Augusta, GA) | |
| Recombinant DNA reagent | pcDNA3.1-Gγ2
(plasmid) |
Hollins et al., 2009 | For the mammalian expression of untagged Gγ2. Provided by N. Lambert (Augusta University, Augusta, GA) | |
| Recombinant DNA reagent | pcDNA3-Gαi3 (plasmid) |
Garcia-Marcos et al., 2010 | For the mammalian expression of rat Gαi3 | |
| Recombinant DNA reagent | pcDNA3.1(-)-Gαi3-YFP | Marivin et al., 2016 | Citrine variant of YFP inserted in the αb/αc loop of Gαi3 | |
| Recombinant DNA reagent | pcDNA3.1-masGRK3ct-Nluc (plasmid) |
Masuho et al., 2015 | Provided by K. Martemyanov (Scripps Research Institute, Jupiter, FL) | |
| Recombinant DNA reagent | pcDNA3.1-mas-KB-1753-Nluc (plasmid) |
Maziarz et al., 2020 | ||
| Recombinant DNA reagent | pcDNA3.1-Nluc-EPAC-VV (plasmid) |
Masuho et al., 2015 | Provided by K. Martemyanov (Scripps Research Institute, Jupiter, FL) | |
| Recombinant DNA reagent | pCS2+−6xMyc-GRK2ct-PM | This paper | Contains bovine GRK2 aa495-689 fused to human Rit aa185-247 See details in ‘Plasmids’ section of 'Materials and methods' |
|
| Recombinant DNA reagent | pcDNA3.1-3xHA-M4R (plasmid) |
cDNA Resource Center at Bloomsburg University | Cat# MAR040TN00 | |
| Recombinant DNA reagent | pcDNA6A-EGFR (plasmid) | Addgene | Cat# 42665 | |
| Recombinant DNA reagent | Grb2-GBA | Parag-Sharma et al., 2016 | Contains Grb2 fused to GIV aa1660-1705 | |
| Chemical compound, drug | NanoGlo Luciferase Assay System | Promega | Cat# N1120 | |
| Chemical compound, drug | Carbachol | Acros Organics | Cat# AC-10824 | |
| Chemical compound, drug | Ramapycin | Alfa Aesar | Cat# J62473 | |
| Chemical compound, drug | Forskolin | Trocis | Cat# 1099 | |
| Chemical compound, drug | Pertussis Toxin | List Biologicals | Cat#179A | |
| Chemical compound, drug | EGF | Gold Biotechnology | Cat# 1150-04-100 |