Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Danio rerio) |
BirA2-GFP | This paper | Tg(cmlc2:birA2-GFP)pd335 | Transgenic line maintained in K. Poss lab |
| Genetic reagent (Danio rerio) | BirA2-GFP-CAAX | This paper | Tg(cmlc2:birA2-GFP-CAAX)pd336 | Transgenic line maintained in K. Poss lab |
| Genetic reagent (Danio rerio) | ErbB2-BirA2-HA-P2A-GFP | This paper | Tg(erbb2-birA2-HA-P2A-GFP)pd337 | Transgenic line maintained in K. Poss lab |
| Genetic reagent (Danio rerio) | DN-RhoA | This paper | Tg(βactin2:loxp-BFP-STOP-loxp-DN-Rho)pd68 | Transgenic line no longer maintained |
| Genetic reagent (Danio rerio) | DN-Rac1 | This paper | Tg(βactin2:loxp-BFP-STOP-loxp-DN-Rac1)pd69 | Transgenic line no longer maintained |
| Genetic reagent (Danio rerio) | DN-Cdc42 | This paper | Tg(βactin2:loxp-BFP-STOP-loxp-DN-Cdc42)pd70 | Transgenic line no longer maintained |
| Genetic reagent (Danio rerio) | CreER | Kikuchi et al., 2010 | Tg(cmlc2:CreER)pd10 | Transgenic line, K. Poss lab |
| Genetic reagent (Danio rerio) | Nrg1 | Gemberling et al., 2015 | Tg(actb2:loxp-TagBFP-STOP-loxp-nrg1)pd107 | Transgenic line, K. Poss lab |
| Genetic reagent (Danio rerio) | Vegfaa | Karra et al., 2018 | Tg(actb2:loxP-TagBFP-loxp-vegfaa)pd262 | Transgenic line, K. Poss lab |
| Genetic reagent (Danio rerio) | CM ablation | Wang et al., 2011 | Tg(actb2:loxp-mCherry-loxp-DipTox)pd36 | Z-CAT cardiac myocyte ablation model, K. Poss lab |
| Antibody | Anti-Mef2 (rabbit polyclonal) | Abcam | Cat#: ab197070 | IF: 1:100 |
| Antibody | Anti-Rho A (rabbit polyclonal) | Santa Cruz | Cat#: 26C4 | IF: 1:1000 |
| Antibody | anti-epb41L5 (rabbit polyclonal) | Proteintech | Cat#: RPL39 | IF: 1:100 |
| Antibody | Anti-Talin 2 (rabbit polyclonal) | Boster Biological Technology | Cat#: PB9961 | IF: 1:100 |
| Antibody | Anti-Seta (rabbit polyclonal) | Antibodies online | Cat#: ABIN2786762 | IF: 1:100 |
| Antibody | Anti-ROCK2 (rabbit polyclonal) | Bioss Antibodies | Cat#: BS-1205R | IF: 1:500 |
| Antibody | Anti-HA (rabbit polyclonal) | Abcam | Cat#: ab9110 | IF: 1:1000 |
| Antibody | Anti-CT3 (mouse monoclonal) | DSHB | Cat#: CT3 | IF: 1:200 |
| Antibody | Anti-PCNA (mouse monoclonal) | Sigma | Cat#: P8825 | IF: 1:200 |
| Antibody | Anti-MHC (mouse monoclonal) | DSHB | Cat#: F59-S | IF: 1:200 |
| Chemical compound, drug | Rhosin | Calbiochem | Cat#: 555460 | 250 μM in 10 μl IP injections |
| Chemical compound, drug | Biotin | Sigma-Aldrich | Cat#: B4501 | 0.5 mM in 10 μl IP injections |
| Chemical compound, drug | EDU | Life Technology | Cat#: A10044 | 100 nM in 10 μl IP injections |
| Chemical compound, drug | Alfacalcidol | Selleck Chemicals | Cat#: S1466 | 200 μM in 10 μl IP injections |
| Recombinant DNA reagent | BirA2-GFP | This paper | cmlc2:birA2-GFP | Plasmid used to generate transgenic line |
| Recombinant DNA reagent | BirA2-GFP-CAAX | This paper | cmlc2:birA2-GFP-CAAX | Plasmid used to generate transgenic line |
| Recombinant DNA reagent | ErbB2-BirA2-HA-P2A-GFP | This paper | cmlc2:erbb2-birA2-HA-P2A-GFP | Plasmid used to generate transgenic line |