Table 1. QC metrics summary for experiments displayed in Figure 1.
Source type | FACS depletion | N pass QC | Median N fragments |
Median mitochondrial reads |
Median unique |
Median in TSS | Median in peaks |
---|---|---|---|---|---|---|---|
Nuclei | Unsorted | 4719 | 7344 | 43 | 0.6% | 5247 | 71.6% | 1332 | 24.9% | 2306 | 43.9% |
Nuclei | Dead/debris | 5526 | 10,526 | 59 | 0.6% | 7284 | 69.4% | 2186.5 | 30.1% | 3647 | 50.5% |
Nuclei | Dead/debris/neutrophils | 7769 | 19,972 | 136 | 0.9% | 11,528 | 59.1% | 4846 | 41.5% | 7503 | 64.8% |
Permeabilized cells | Unsorted | 6329 | 5541 | 100 | 1.9% | 3308 | 59.8% | 871 | 26.4% | 1390 | 42.2% |
Dead/debris | 6956 | 6733.5 | 120 | 2.0% | 3795.5 | 56.8% | 1219.5 | 32.6% | 1874 | 50.2% | |
Dead/debris/neutrophils | 9849 | 14,069 | 514 | 4.0% | 4756 | 34.3% | 2536 | 54.4% | 3650 | 76.8% | |
For all metrics to the right of median N fragments, both the absolute number and a percentage are provided. Median % mitochondrial and median % unique were calculated as a fraction of total fragments; % in TSS and % in peaks were calculated as a fraction of unique fragments. TSS: transcription start sites; FACS: fluorescence-activated cell sorting. |