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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Clin Cancer Res. 2020 Nov 25;27(1):276–287. doi: 10.1158/1078-0432.CCR-20-2291

Figure 4. Concurrent dexamethasone increases apoptosis of CD4+ and CD8+ T cells in the GL261-luc2 glioblastoma mouse model.

Figure 4.

(A) Late apoptosis was evaluated by 7-AAD+ and annexin-V+ staining in non-tumor-bearing mouse spleens (n=3/group, from a single experiment) either 1 hour after the first dexamethasone dose or 1 hour after the sixth daily dexamethasone dose. Apoptosis differences were tested by two-way ANOVA with post-test correction. Cell counts normalized to the corresponding IgG control group’s mean count (B) and percent (C) of proliferating CD4+ and CD8+ T cells were evaluated by Ki67 staining, using the same dosing schema and analyses as Figure 3 (n=4–8/group, derived from two experiments).

cLN, cervical lymph node; Dex, dexamethasone; hr, hour; ns, not significant, p≥0.05; *p<0.05; **p<0.01; ***p<0.001