Fig. 1.

The jSR in rabbit ventricular myocytes is wider in HPF preserved cells, compared to CFD. A: Comparison of jSR (red arrows) in representative TT-perpendicular ET slices extracted from 3D volumes of rabbit ventricular (coronary perfusion-) chemically fixed and dehydrated tissue (tissue CFD), isolated CFD-preserved cells (cell CFD), and high-pressure frozen isolated cells (cell HPF, same batch as cell CFD). B: jSR width at its widest projection (red arrow) in rabbit cardiomyocytes is highest in cells preserved by HPF when compared both to isolated cells or tissue, preserved by CFD; ***p < 0.001; one-way ANOVA with Bonferroni's post-hoc test; n = 20 TT-jSR pairs/ 16–20 cells/ 3 hearts. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)