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. 2021 Apr 9;11:7834. doi: 10.1038/s41598-021-87324-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The SC of EVR and OR dogs show strikingly different types of biochemical changes. (a) Principal component analysis showing a clear separation between the samples from EVR dogs #1–3 and the clustered samples from control and OR dogs. There is also evidence of a more limited separation between the samples from thoracic and lumbar spinal cord. (b)–(p) Box and whisker plots showing the scale intensity for representative metabolites in the thoracic (T) and lumbar (L) SC of control dogs (n = 4), OR dogs (n = 4) and EVR dogs #1–3 (E1, E2 and E3, respectively). Results are presented separately for the three EVR dogs to illustrate the consistency of data between them, irrespective of the extent of stenting. Statistical analyses, however, were conducted using the three EVR dogs as a group. Significance was assessed using a Welch’s two-sample t-Test (p < 0.05).