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. 2021 Apr 9;12(4):385. doi: 10.1038/s41419-021-03671-2

Fig. 1. HuR levels were reduced in atherosclerotic plaque.

Fig. 1

A, B Western blot analysis of HuR protein level in aortas from apolipoprotein E-knockout (ApoE−/−) mice fed a high-fat diet (HFD) or normal diet (ND) (n = 5). C Immunohistochemical staining of HuR in aortas from ApoE−/− mice fed an HFD or ND (n = 5). D Quantitative RT-PCR analysis of HuR mRNA level in aortas from ApoE−/− mice fed an HFD or ND (n = 5). E, F Western blot analysis of HuR protein level in vascular smooth muscle cells (VSMCs) induced by 50 μg/ml oxidized low-density lipoprotein (ox-LDL) at different times (n = 5). G, H Western blot analysis of HuR protein level in aortas from control and HuRSMKO mice (n = 5). I Immunofluorescent staining of aortas from control and HuRSMKO mice to determine HuR (green) and α-SMA (α-smooth muscle actin; red) localization. Scale bar = 50 μm.