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. 2021 Apr 9;12(4):385. doi: 10.1038/s41419-021-03671-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Immunofluorescent staining of aortic roots from CTR and HuR^SMKO mice to determine TUNEL-positive VSMCs. Red puncta denotes TUNEL-positive cells. Green region denotes α-SMA. Scale bar = 20 μm. B Immunohistochemical staining of cleaved caspase-3 in aortic roots (n = 6). Scale bar = 50 μm. C Serum lipid profiles (total cholesterol [TC], triglycerides [TG], high-density lipoprotein cholesterol [HDL-C], and low-density lipoprotein cholesterol [LDL-C]) (n = 6). D Western blot analysis of cleaved caspase-3 in control and HuR-deficient SMCs (n = 5).