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. 2021 Apr 9;11:7815. doi: 10.1038/s41598-021-87382-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PQE Atx7 and Htt reduce the soluble fraction of intracellular Atx3 but increase the insoluble. (A) Effects of cytoplasm-localized Atx7_93Q-N172 on the supernatant and pellet fractions of Atx3. (B) As in (A), cytoplasm-localized Htt_100Q-N90. (C) As in (A), nucleus-localized NLS-Atx7_93Q-N172. HEK 293T cells were transfected with each indicated plasmid and the cell lysates were subjected to supernatant/pellet fractionation and Western blotting analysis. Indicated proteins were detected by using anti-FLAG, anti-Atx3 and anti-GAPDH antibodies. The two main bands indicate different isoforms of endogenous Atx3, Atx3-I and Atx3-II. Sup., supernatant; Pel., pellet. The gray values of Atx3 protein bands from three independent experiments were employed for statistical analysis. Data are shown as Means ± SEM (n = 3). **p < 0.01; ***p < 0.001.