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. 2021 Apr 9;11:7818. doi: 10.1038/s41598-021-87301-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental SAH neurobehavioural and histological assessments. (a) Mortality after SAH induction or sham procedure. (b) Modified Garcia Score at 24 h and 48 h after SAH induction or sham procedure (maximum normal score is 18). (c) Merged triple-stained immunofluorescent confocal microscopy of coronal brain slices 24 h after SAH induction or sham procedure (NeuN (neuronal marker) = red, Caspase-3 (apoptosis marker) = green, DAPI (nuclei marker) = blue). Arrows point to apoptotic neurons. (d) Semi-automated apoptotic neuronal count from left ventral region of coronal brain slices. (e) Fluorescent confocal microscopy with fluoro-jade B staining of coronal brain slices 24 h after SAH induction or sham procedure. Arrows point to degenerated neurons. (f) Semi-automated degenerated neuronal count from left ventral region of coronal brain slices. n = 8–11 per group for 1a-b. n = 4–5 per group for 1c-f. Data presented as means ± SEM (Standard error of the mean). Kruskal–Wallis test (Modified Garcia Score). t-test (Caspase 3, fluoro-jade B). *p < 0.05, **p < 0.01.