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. 2021 Apr 9;11:7818. doi: 10.1038/s41598-021-87301-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Celecoxib treatment after experimental SAH. (a) Whole brain fluorescent imaging from ventral perspective 24 h after SAH or sham procedure, treated with selective Cox2 inhibitor celecoxib or vehicle. Left ventral region demarcated by yellow marking. Cadaverine dye fluorescence shown in red. (b) Quantification of cadaverine dye fluorescence in left ventral region. (c) Merged double-stained immunofluorescent confocal microscopy of coronal brain slices 24 h after SAH treated with celecoxib or vehicle (NeuN (neuronal marker) = red, Caspase-3 (apoptosis marker) = green). Apoptotic neurons noted in yellow. (d) Semi-automated apoptotic neuronal count from left ventral region of coronal brain slices. n = 3–4 per group. Data presented as means ± SEM. One-way ANOVA with Holm-Sidak post-hoc correction. *p < 0.05.