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. 2021 Apr 9;7:73. doi: 10.1038/s41420-021-00463-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Results of 5′ RACE and 3′ RACE. M, DL2000 Marker; 5′ RACE product, 690 bp; 3′ RACE product, 1465 bp. B Analysis obtained from the Coding Potential Calculator (http://cpc.cbi.pku.edu.cn/) based on evolutionary conservation and ORF attributes. C LncRNA-FKBP1C is localized in the cytoplasm and nucleus of chicken primary myoblasts. GAPDH and U6 serve as cytoplasmic and nuclear localization controls, respectively. D XH chicken tissue expression profiles of lncRNA-FKBP1C. Cer, cerebrum; Ceb, cerebellum; Hyp, hypothalamus; Pit, pituitary; Hea, heart; Liv, liver; Spl, spleen; Lun, lung; Kid, kidney; Brm, breast muscle; Lem, leg muscle. E Western blot analysis of the coding ability of lncRNA-FKBP1C. The possible ORF of lncRNA-FKBP1C was cloned into the eukaryotic expression vector pcDNA3.1-3xFlag. Untransfected DF-1 cells were used as a negative control (NC) and DF-1 cells transfected with pcDNA3.1-3xFlag-β-actin were used as a positive control. The upper panel shows the model target fragment in the pcDNA3.1-3xFlag vector. The lower left panel is the blot of ORFs 1–11, and the lower right panel is the blot of the NC and β-actin. F The location of 11 potential ORFs in lncRNA-FKBP1C. 11 potential ORFs are arranged in order according to their positions. Results are shown as mean ± S.E.M. and the data are representative of at least three independent assays. Independent sample t-test was used to analyze the statistical differences between groups (*P < 0.05, ***P < 0.001).