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. 2021 Apr 9;7:73. doi: 10.1038/s41420-021-00463-7

Fig. 5. LncRNA-FKBP1C interacts directly with MYH1B and increases the protein level of MYH1B by enhancing its protein stability.

Fig. 5

A RNA pulldown silver stained image and binding proteins screened by mass spectrometry. Red box indicates MYH1A, MYH1B and MYH11 proteins; yellow box indicates TPM1 and TPM3 proteins. B Prediction of the interaction between LncRNA-FKBP1C and MYH1B by using catRAPID (http://service.tartaglialab.com/page/catrapid_group). C, D Interaction between LncRNA-FKBP1C and MYH1B as determined by biotin-labeled RNA pulldown and RIP. E The qPCR result of RIP. F, G The mRNA and protein expression of MYH1B induced by LncRNA-FKBP1C overexpression and interference in CPM cells (N.S. is the abbreviation for not significant). H The CPM cells after 48 h transfected with pcDNA3.1-lncRNA-FKBP1C and pcDNA3.1 incubated with the protein synthesis inhibitor CHX (1 mg/mL) for 0, 3 or 6 h. I The CPM cells after 48 h transfected with si-ASO-lncRNA-FKBP1C and si-NC incubated with the protein synthesis inhibitor CHX (1 mg/mL) for 0, 3 or 6 h. J The CPM cells after 48 h transfected with si-ASO-lncRNA-FKBP1C and si-NC incubated with MG-132 (100 μmol/L) for 0, 3 or 6 h. K The CPM cells after 48 h transfected with pcDNA3.1-lncRNA-FKBP1C and pcDNA3.1 incubated with MG-132 (100 μmol/L) for 0, 3 or 6 h.