Fig. 4. Inhibition of distinct NAc glutamatergic inputs regulates high fat place preference.

a Schematic of experimental setup and design. b Schematic of viral injection and ferrule implant for aPVT input inhibition. c Schematic of viral injection and ferrule implant for PFC input inhibition. d Trajectory of a typical animal during test that received aPVT input inhibition during the conditioning phase. e, f Quantification of time spent in the paired chamber (e: F_1,15 = 6.948, P = 0.02, n = 10,7 mice/group) and CPP score for paired chamber (f: t = 2.636, P = 0.02, n = 10,7 mice/group) in eYFP control (black) and NpHR expressing (green) mice. g Trajectory of a typical animal during test that received PFC input inhibition during the conditioning phase. h, i Quantification and time spent in paired chamber (h: F_1,14 = 5.843, P = 0.03, n = 9, 7 mice/group) and CPP score for paired chamber (i: t = 2.417, P = 0.03, n = 9, 7 mice/group) in eYFP control (black) and NpHR expressing (green) mice. Data are ±s.e.m. *P < 0.05, e, h two-way ANOVA with Sidak’s multiple comparison post hoc test, f, i Student’s t test. Source data are provided as a Source Data file.