Fig. 4. Analysis of AA7-LPMO interplay in cellulose degradation.

Reactions were performed on Avicel using FgCelDH7C and PaLPMO9H with subsequent ionic chromatography (HPAEC-PAD) analysis. a A representative chromatogram part showing C1-C4 double-oxidised species in the cellulose degradation assay including combinations of Avicel (5 mg mL⁻¹), FgCelDH7C (0.4 μM), PaLPMO9H (4 μM), PaCDHB (1.2 µM), cellotetraose (DP4, 0.8 mM) and ascorbate (Asc., 1 mM) as indicated in the figure. b Comparison of the cellulose degradation assay based on the cumulative area under the peaks of native (DP3, DP5 and DP6), C1 oxidised (except DP2 and DP4 which were added as substrates for the CDH and AA7, respectively) and C1-C4 double-oxidised cellooligosaccharides from the reactions in (a). The data in (a) and (b) (n = 3 independent reactions) were generated in NaOAc/NaOH buffer (50 mM, pH 5.2) at 35 °C. The bar plot in (b) shows the means of total peak area (n = 3 independent reactions, each shown as a white circle) with standard deviations. Source data are provided as a Source Data file.