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. 2021 Apr 9;7:40. doi: 10.1038/s41523-021-00248-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Profiling of the drug-able kinome by MIB/MS analysis in HCC1143 cells identified 18 kinases with significantly reduced expression and/or affinity for Type I kinase inhibitor bait (p < 0.05) and 12 kinases with increased expression/affinity following siRNA-mediated silencing of SOX4 (n = 3, 96 h) relative to siControl-treated samples (n = 3); kinase enrichment score is shown in yellow and kinase depletion score in blue. b Differential gene expression analyses of RNAseq data for the significantly altered kinases from MIB/MS analysis from siSOX4- and siControl-treated HCC1143 cells (96 h); high mRNA expression is shown in red and low expression in green. c HCC1143 and d HCC1954 cells demonstrate significant reduction (unpaired t-test) in SOX4 (p < 0.0001; p = 0.019) and TGFBR2 (p = 0.014; p = 0.012) expression in siSOX4-treated cells compared to siControl-treated cell by qRT-PCR; data are presented as mean ± SD and normalized for GAPDH (n = 3). e HCC1143 and f HCC1954 cells showing reduced SOX4 and TGFBR2 protein expression by western blot analyses in siSOX4-treated cells relative to siControl-treated cells.