Fig. 5. SOX4 and SMARCA4 form a complex at TGFBR2 promoter and enhancer region.
a Potential interacting partners of SOX4 from the SWI/SNF chromatin remodeling complex family of proteins identified by LC-MS/MS. Spectral counts for IgG and V5 antibody for each protein is listed. b mRNA expression patterns of SWI/SNF complex core subunit genes relative to SOX4 were determined for 1031 human breast tumors and 94 adjacent normal samples from the TCGA dataset. Samples are organized by PAM50 molecular subtype; red indicates high mRNA expression, and blue depicts low mRNA levels. c Co- immunoprecipitation of SOX4 (V5) and SMARCA4 followed by reciprocal western blot analyses demonstrates the interaction between these proteins in HCC1143SOX4-V5 cells; results are representative of three independent experiments. d HEK293T cells were lentivirally overexpressed with HA-SOX4 and co-immunoprecipitation performed with HA antibody followed by western blot detection of SMARCA4 demonstrating the interaction between these proteins. e SMARCA4 ChIP-qPCR in HCC1143 demonstrated a 3.6- and 6.6-fold enrichment of SMARCA4 at the TGFBR2 promoter (p = 0.0024; unpaired t-test) and enhancer (p = 0.0008; unpaired t-test) respectively. f SMARCA4 ChIP-qPCR in HCC1954 cells demonstrated a 2.7- and 1.8-fold enrichment of SMARCA4 at the TGFBR2 promoter (p < 0.0001; unpaired t-test) and enhancer (p = 0.0002; unpaired t-test), respectively.