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. 2021 Apr 9;7:40. doi: 10.1038/s41523-021-00248-2

Fig. 6. SOX4 recruits SMARCA4 to remodel chromatin and mediates TGFBR2 expression.

Fig. 6

ChIP-qPCR demonstrating reduction in SMARCA4 enrichment following siRNA-mediated knockdown of SOX4 by 70.49% (p = 0.001; unpaired t-test) and 57.9% (p = 0.0007; unpaired t-test) in HCC1143 (a) and by 61.49% (p = 0.0007; unpaired t-test) and 62.8% (p = 0.006; unpaired t-test) in HCC1954 (b) cells at the TGFBR2 promoter and enhancer regions relative to the scrambled control. Western blot analysis demonstrating siRNA-mediated silencing of SOX4 results in decreased SMARCA4 protein expression in HCC1143 (c) and HCC1954 (e) cell lines. ChIP–western blot analysis demonstrating equivalent levels of SMARCA4 protein pulldown in cells treated with either siRNA targeting SOX4 or control siRNA in HCC1143 (d) and HCC1954 (f) cell lines. Chromatin accessibility assay demonstrating that accessibility was reduced following siRNA-mediated knockdown of SOX4 by 39.7% (p = 0.015; unpaired t-test) and 26.5% (p = 0.0005; unpaired t-test) in HCC1143 (g) and by 36.9% (p = 0.03; unpaired t-test) and 33.4% (p = 0.017; unpaired t-test) in HCC1954 (h) cells at the TGFBR2 promoter and enhancer regions, respectively, relative to the scrambled control. qRT-PCR analysis demonstrating decreased TGFBR2 mRNA expression in HCC1143 (i) and HCC1954 (j) cell lines following RNAi-mediated silencing of SMARCA4. Representative western blot analysis demonstrating loss of TGFBR2 and pAkt protein expression in HCC1143 (k) and HCC1954 (l) cell lines following RNAi-mediated silencing of SMARCA4.