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. Author manuscript; available in PMC: 2021 Oct 5.
Published in final edited form as: Nat Cell Biol. 2021 Apr 5;23(4):366–376. doi: 10.1038/s41556-021-00646-5

Extended Data Figure 1: Fcho2 assembles into protein-rich domains with Eps15 on membranes and in solution.

Extended Data Figure 1:

(a-e) Fcho2 is labeled with Atto-594. Eps15 contains an N-terminal 6xHis tag and is labeled with CF488a. (a-b) Center slices (upper panels) and corresponding z-projections (lower panels) of representative GUVs incubated with 500 nM of the indicated protein(s). GUVs contain 79% DOPC, 15% DOPS, 5% PtdIns(4,5)P2, and 1% DPEG10-biotin. (a) Full-length Fcho2 alone decorates GUVs homogeneously. (b) GUVs incubated with both Fcho2 and Eps15 display protein-rich domains. (c) 7 μM Fcho2 clusters into small, irregular aggregates. (d) When combined at a 1:34 ratio, Fcho2 and Eps15 colocalize in protein droplets. (e) Time course of a fusion event between droplets containing Fcho2 (magenta) and Eps15 (green). Scale bars are 5 μm in a-c, e and 10 μm in d.