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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Gastroenterology. 2021 Jan 6;160(5):1771–1783.e1. doi: 10.1053/j.gastro.2020.12.077

Fig. 6. ZIP4 promotes EMT in pancreatic cancer.

Fig. 6.

(A). H&E staining of 3D tumor spheroid generated from MIA-V and MIA-ZIP4 cells. Spheroids were collected after 3 days of spheroid culture and processed tissue embedding and sectioning. The scale bar is 100 μm. (B). Expression of EMT markers Vimentin, N-cadherin, E-cadherin or claudin-1 was examined in AsPC-shV and AsPC-shZIP4 cells cultured under 2D and 3D conditions. (C). Expression of EMT markers in MIA-V and MIA-ZIP4 cells. (D). Protein levels of LATS2, YAP1 and ITGA3 in AsPC-shV and AsPC-shZIP4 cells cultured under 2D and 3D conditions. (E). Protein levels of LATS2, YAP1 and ITGA3 in MIA-V and MIA-ZIP4 cells. (F). Schematic diagram of the signaling axis of ZIP4-miR-373-LATS2-ZEB1/YAP1/TEAD-ITGA3.