Extended Data Figure 1. Controls for two-choice odor preference assay.

a, Timeline of two-choice behavioral assay. b-c, Odor investigation times of fed (b) and fasted (c) male and female wild type mice in single odor pairings with water. (n=10 males and 10 females for b, 3 males and 3 females for c, mean ± s.e.m, lines with triangles: individual mice, *p<.05, ***p<.001 by two-tailed Wilcoxon test (p left to right in b: <.0001, <.0001, .86; p left to right in c: .03, .03). A statistical comparison of odor responses in b and c using a two-tailed Mann-Whitney U test revealed significant increases in food odor investigation in fasted mice compared with fed mice (p=.0006) but not in pheromone investigation (p=.15). d, Odor investigation times of fed (left) and fasted (right) wild type females, n=10 mice, mean ± s.e.m, lines with triangles: individual mice, **p<.01 by two-tailed Wilcoxon test (p fed: .77, p fasted: .002), e, Odor investigation times of male (left) and female (right) wild type mice fed ad libitum, n=10 mice, mean ± s.e.m, lines with triangles: individual mice, p values by two-tailed Wilcoxon test: male=.92, female=.19.