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. Author manuscript; available in PMC: 2022 Apr 7.
Published in final edited form as: Neuron. 2021 Feb 17;109(7):1137–1149.e5. doi: 10.1016/j.neuron.2021.01.028

Figure 1. Partial depletion of striatal dopamine following low-dose 6-OHDA induces mild motor impairment without altering ChI pacemaker firing.

Figure 1.

(A) Schematic and timeline of 6-OHDA injection.

(B) Coronal striatal section of TH-immunoreactivity following low dose (1 μg/ μL) unilateral 6-OHDA injection.

(C) TH-immunoreactivity intensity of injected hemisphere relative to contralateral saline injected hemisphere. N = 4.

(D) Coronal midbrain section showing the loss of TH-immunoreactivity in the SNc following low dose (1 μg/ μL) unilateral 6-OHDA injection.

(E) TH-immunoreactivity intensity of injected hemisphere relative to contralateral saline injected hemisphere. N = 5.

(F) Peak striatal FSCV [DA]o from saline and low-dose 6-OHDA injected hemispheres. Dopamine evoked with a single electrical stimulus (1 ms). Left: illustration of the FSCV waveform. N = 6 for both groups.

(G) Contralateral paw use in cylinder behavioral test. N = 12 – 16.

(H) Cell attached recordings of DMS and DLS ChIs from control and low-dose 6-OHDA (1 μg / μL) groups.

(I) Quantification of cell attached baseline pacemaker firing rates. n = 8 - 16. N = 3 - 6. Summary data are mean ± SEM. ** = p < 0.01, *** = p < 0.001, n.s = p > 0.05.