KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-TH | Millipore | Cat# AB152 |
| Donkey anti-rabbit Alexa Fluor 488 | Life Technologies | Cat# A21206 |
| Rabbit anti-DDDDK tag | Abcam | Cat# ab1162 |
| Donkey anti-rabbit Alexa Fluor 594 | Abcam | Cat# ab150076 |
| Bacterial and Virus Strains | ||
| AAV5.EF1a.DIO.hChR2 (H134R)-EYFP | pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA was a gift from Karl Deisseroth | Addgene plasmid: 20298 |
| AAV9.hSyn.tdTomato.T2A.mGIRK2-1 | Marcott et al., 2014 | Penn Vector Core: V3992MI-R |
| AAV5-hSyn-DIO-hM3D-mCherry | Krashes et al., 2011 | Addgene plasmid: 44361 |
| AAV5-EF1a-mCherry-Cre | UNC Vector Core | AV6144B |
| AAVDJ-DIO-mGluR1-myc-FLAG | This manuscript | Addgene plasmid: 166227 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Alexa Fluor 594 | Thermo Fisher | Cat# A10436 |
| Cal520 | ATT Bioquest | Cat# 21141 |
| 6-hydroxydopamine hydrobromide | Tocris | Cat# 2547 |
| Desipramine hydrochloride | Tocris | Cat# 3067 |
| Pargyline hydrochloride | Sigma | Cat# P8013 |
| DNQX | Tocris | Cat# 0189 |
| Picrotoxin | Tocris | Cat# 1128 |
| MK-801 | Tocris | Cat# 0924 |
| JNJ16259685 | Tocris | Cat# 2333 |
| L-aspartate | Tocris | Cat# 0214 |
| Sulforhodamine 101 | Sigma | Cat# S7635 |
| Sulpiride | Tocris | Cat# 0895 |
| Clozapine N-oxide | Tocris | Cat# 4936 |
| (RS)-3,5-DHPG | Tocris | Cat# 0342 |
| Substance P | Tocris | Cat# 1156 |
| Bafilomycin A1 | Tocris | Cat# 1334 |
| BAPTA | Acros Organic | Cat# 123311000 |
| Critical Commercial Assays | ||
| PerfeCTa ToughMix Low Rox | Quantabio | Cat# 95112 |
| SMART-Seq HT kit | Takara | Cat# 634455 |
| Experimental Models: Cell Lines | ||
| HEK-239T cells | ATCC | Cat# 11268 |
| Experimental Models: Organisms/Strains | ||
| Mouse / DAT-IRES-Cre | Jackson Labs | JAX: 006660 |
| Mouse / ChAT-IRES-Cre | Jackson Labs | JAX: 006410 |
| Mouse / Floxed VGLUT2 | Jackson Labs | JAX: 007583 |
| Mouse / CaMKII-Cre | Jackson Labs | JAX: 005359 |
| Mouse / C57Bl6 | Jackson Labs | JAX: 000664 |
| Oligonucleotides | ||
| B-actin | IDT | mmPT: 39a.2214843.g |
| Chat | IDT | mmPT: 58.31626536 |
| Ctip2 | IDT | mmPT: 58.31107142 |
| Grm1 | IDT | mmPT: 58.8395588 |
| Recombinant DNA | ||
| mGluR1-Myc/DDK | Origene | Cat# MR211136 |
| Software and Algorithms | ||
| Axograph X | Axograph Scientific | RRID SCR_014284 https://axograph.com |
| Prism | GraphPad Software | RRID SCR_002798 https://www.graphpad.com/scientific-software/prism |
| Toronado | Pressler & Strowbridge, 2019 | https://github.com/StrowbridgeLab/Toronado-Laser-Scanning |