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. Author manuscript; available in PMC: 2022 Apr 12.
Published in final edited form as: J Chromatogr A. 2021 Mar 9;1642:462047. doi: 10.1016/j.chroma.2021.462047

Figure 4.

Figure 4.

Comparison of the average peak volume from methods chosen for scale down to microflow-based LC separations in the A) positive ESI mode and B) negative ESI mode. The standard mixture (100 ng/mL) was separated on 0.3 × 100 mm columns with a column temperature of 40 °C using a 30 min LC method. The injection volume was 1 μL. Data points and error bars represent average peak volume results and standard deviation for three replicate analyses. The scale of the X-axis is log 10. FA: formic acid mobile phases; AmAc: ammonium acetate mobile phases; Mixed: mixed-mode methods; AmF: Ammonium formate; NH4F: Ammonium fluoride