Extended Data Fig. 5. METTL3 and METTL14 promote SASP.

a-d, IMR90 cells ectopically expressing METTL3, wildtype or a R298P mutant METTL14 were subjected to analysis for expression of the indicated proteins by immunoblots (a), colony formation assay (b), SA-β-gal staining (c) or expression of the indicated SASP genes by qRT-PCR (d). The experiment in 5a was repeated twice independently with similar results. e, IMR90 cells expressing oncogenic RAS with or without ectopically expressed wildtype or the R298P mutant METTL14 were subjected to qRT-PCR analysis for expression of the indicated SASP genes. f, IMR90 cells with or without expressing the indicated wildtype or mutant METTL3 or METTL14 were harvested at day 6 post infection and analyzed for expression of the indicated proteins by immunoblot. The experiment was repeated twice independently with similar results. g-h, Conditioned medium collected from senescent cells with the indicated inducers were used to culture proliferating cells for 5 days. Changes in SA-β-gal (g) and BrdU incorporation (h) were examined. Data represent mean ± s.d. of three biologically independent experiments. P values were calculated using a two-tailed t-test. Uncropped blots for 5a and 5f and numerical source data for 5b, 5c, 5d, 5e, 5g and 5h are provided.