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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Cell Biol. 2021 Apr 1;23(4):355–365. doi: 10.1038/s41556-021-00656-3

Extended Data Fig. 10. MTC is required for immune surveillance function of the SASP.

Extended Data Fig. 10

a, Validation of METTL3 and METTL14 knockdown by qRT-PCR analysis in mouse NIH3T3 cells. n=3 biologically independent experiments. b, Validation of METTL3 and METTL14 knockdown by immunofluorescence analysis in mouse NIH3T3 cells. Arrows point to dsRed-expressing shRen control, shMETTL3 or shMETTL14. Bar = 10 μm. The experiment was repeated two times independently with similar results. c, Immunohistochemical staining of NRas in liver tissues injected with a mutant NRasV12/D38A that is incapable of inducing senescence at day 6. The experiment was repeated in 3 biologically independent mice with similar results. Bar = 50 μm. d, Quantification of CD45+/NRas+ foci in the livers isolated from the indicated mice at day 6. n = 6 biologically independent mice per group. Data represent mean ± s.d. P values were calculated using a two-tailed t-test. Numerical source data for 10a and 10d are provided.