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. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Nat Cell Biol. 2021 Apr 1;23(4):355–365. doi: 10.1038/s41556-021-00656-3

Figure 4: METTL3 is localized to the pre-existing NF-κB sites within the promoters of SASP genes.

Figure 4:

a, Enrichment of SASP genes among genes whose promoters showed an increased association with METTL3 in senescent cells. b, Heatmap of binding signal around METTL3 peaks found in control and RAS-induced senescent cells from cut-and-run seq for METTL3 and NF-κB p65. c, Co-immunoprecipitation analysis between METTL3 or METTL14 and NF-κB p65 subunit and RNA polymerase II in control and RAS-induced senescent cells. The experiment was repeated three times independently with similar results. d-e, ChIP–qPCR analysis of the association of METTL3 and NF-κB p65 with the promoters of the indicated SASP genes in control and RAS-induced senescent cells with or without METTL3 or METTL14 knockdown or treated with IKK inhibitor Bay 11–7082 (5 μM) for 48 hrs. Data represent mean ± s.e.m. of three biologically independent experiments. P values were calculated using a two-tailed t-test except in 4a by a two-tailed Fisher Exact test. Uncropped blots for 4c and numerical source data for 4d and 4e are provided.