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Total cumulative sprout length of vascular sprout formation in response to imposed gradients after 7 days (A), 14 days (B) and fold increase from day 7 to day 14 (C). 3D volume rendering of phalloidin-stained spheroids encapsulated in top (D), middle (E) and bottom (F) regions of steep RGD gradient hydrogel at day 7. Error bars represent standard deviation. Asterisks (*) indicate statistical significance with p < 0.05 (n=3).
