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. 2021 Apr 9;51:4. doi: 10.1186/s42649-021-00053-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Segmentation and coordinates representation of signals to determine 3D co-localization, break-apart and other variations in individual cell nuclei patterns. a Volume and segmentation representation. Showing Z-stack images scanned with 7-layer and 0.6 μm interval at the same area. Blue is DAPI channel for stained nuclei, green is FITC channel, and red is TRITC channel. b 3D representation of selected volume from panel A. c Segmentation of clear individual cell nuclei (shown in gray) found at the volume. d Coordinates representation of segmented signals from the 7-layers Z-stack. e 3D vector length calculation using the X, Y and Z coordinates extracted from the 7-layers Z-stack (to determine co-localization and break-apart between FITC and TRITC signals) and classification of cell nuclei pattern. f Collection of segmented individual cell nuclei to show variations in signals patterns (normal in orange frame, break in purple frame and other patterns in gray frame)