Fig. 1. scRNA-seq analysis of isolated mouse and human glomerulus-associated cells.

a Summarized study design and experimental procedure. b Projection of mouse and human single cells onto 2-D UMAP space coloured by assigned cell types. Cell numbers in each population are shown in parenthesis. c The expression of known cell type marker genes in mouse and human principal glomerular cells. The markers include Nphs1/NPHS1 for podocytes, Pdgfrb/PDGFRB for mesangial-like cells (MLCs) and Pecam1/PECAM1 for ECs. The colour intensity of dots represents the expression level. The colour scale is defined by log₂(mean RPKM). d Mouse and human podocyte, glomerular EC, and MLC enriched active regulons constructed by SCENIC. Regulons active in >50% of cells in one cell type but not in other two cell types were considered as cell-type enriched regulons. Binary activity scores are shown in the heatmap with black indicating ‘ON/active’ and white indicating ‘OFF/inactive’. CD collecting duct, DCT distal convoluted tubule, PTC proximal tubule cell, EC endothelial cell, MLC mesangial-like cell, MNP mononuclear phagocyte, PEC glomerular parietal epithelial cell, cTAL cortical thick ascending limb; colour dots indicate diverse cell types.