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. 2021 Apr 9;12:2141. doi: 10.1038/s41467-021-22331-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The expression (mean log₂-transformed RPKM) of mouse and human gene homologues in MCs. Human- (n = 40) and mouse-specific (n = 70) genes are indicated in salmon and light blue, respectively. Conserved genes highly expressed in both species (n = 243) are indicated in red. Detailed information can be found in Supplementary Data 8. b The expression of conserved MC overexpressing genes in single-cell data generated from C57BL/6J mice with viable CD45⁻ CD31⁻ cell sorting (left), Pdgfrb-EGFP mice (middle) and human donor biopsies (right). The colour intensity and size of each dot represents the mean expression (standard scale) and the percentage of cells expressing each gene in individual cell types, respectively. c Heatmap showing the expression of differentially expressed genes in MCs between mouse and human. The colour scale is defined by log₂(mean RPKM). d Validation of mouse-specific desmin expression in MCs. Immunofluorescence staining for desmin (red) shows abundant expression in the mouse glomerulus (arrowheads). Expression of desmin is undetectable in human glomeruli (www.proteinatlas.org, https://creativecommons.org/licenses/by-sa/3.0/), but strong signal in vSMCs (arrowheads) is detected as a positive control. Scale bars are indicated in images. e Validation of human-specific COL6A1 expression in MCs. COL6A1 (red) is detected in human MCs (arrowheads), whereas in mouse no significant reactivity in glomeruli is observed. PDGFRB (green) labels MCs in the glomerulus. Abundant extraglomerular interstitial reactivity for COL6A1 (arrowheads) serves as a positive control in mouse. Discontinuous circles indicate glomeruli. Scale bars: 15 µm for mouse and 35 µm for human.