Table 1.

Recombinant proteins produced in tobacco BY-2 cell cultures

Protein	Expression type	Protein targeting	Protein yield	Reference
Hepatitis B surface antigen	Stable	ER	226 ng/mg TSP	Sojikul et al. (2003)
Human monoclonal antibody against hepatitis B virus	Stable	Apoplast	0.6% TSP	Yano et al. (2004)
Human interferon α2b	Stable	Apoplast	35 mg/L	Xu et al. (2007)
Human α -l-iduronidase	Stable	Apoplast	10 mg/L	Fu et al. (2009)
Human monoclonal anti-HIV antibody 2G12	Stable	Apoplast	8 mg/L	Holland et al. (2010)
Human growth hormone	Stable	Apoplast	28 mg/L	Xu et al. (2010)
Human serum albumin	Stable	Cytosol, Vacuole	11.88 mg/L	Sun et al. (2011)
Human erythropoietin	Stable	ER, Apoplast	N/A	Pires et al. (2012)
Human monoclonal anti- vitronectin antibody M12	Stable	Apoplast	20–107 mg/L	Kirchhoff et al. (2012), Vasilev et al. (2013), Raven et al. (2015)
Human interleukin-10-ELP (elastin‐like polypeptide)	Stable	ER	3.057% TSP	Kaldis et al. (2013)
Green fluorescent protein (GFP)	Stable	Apoplast	125 mg/L	Zhang et al. (2016a, b)
TNFα receptor (TNFR)-Fc fusion	Stable	ER	N/A	Almon et al. 2017), Ilan et al. (2017)
DNase I	Stable	Apoplast	N/A	Hanania et al. (2017)
GFP-hydrofobin (HFBI)	Stable	Apoplast	300–1100 mg/L	Reuter et al. (2014, 2016), Hakkinen et al. (2018)
Human α1-antitrypsin	Stable	Apoplast	34.7 mg/L	Zhang et al. (2019)
ORF8 from SARS-CoV-2	Stable (inducible)	Apoplast	8.8 mg/L	Imamura et al. (2021)
GFP	Stable (inducible)	Apoplast	N/A	Sadoch et al. (2021)
Human stem cell factor	Stable	Apoplast	2.5 mg/L	Wang et al. (2021)
Viscumin	Transient	Apoplast	5.0 mg/kg cells	Gengenbach et al. (2019)
DsRed (Discosoma sp. red fluorescent protein)	Transient	Apoplast	70 mg/kg cells	Rademacher et al. (2019)
Monoclonal antibody 2G12			47 mg/kg cells
Monoclonal antibody M12			175 mg/kg cells
GFP and DsRed	Transient	Apoplast	~ 700 mg/kg cells	Poborilova et al. (2020)

N/A not available, TSP total soluble proteins