Skip to main content
. 2021 Apr 9;41(4):BSR20201738. doi: 10.1042/BSR20201738

Figure 5. Knockdown of GAS5 inactivates the HMGB1/NF-κB pathway in mice with sepsis.

Figure 5

LV-GAS5-shRNA or LV-GAS5 was used to change GAS5 expression in septic mice and HMGB1 was overexpressed in septic mice with reduced GAS5 expression. (A) The protein level and the phosphorylation of p65 in septic mice after GAS5 and HMGB1 intervention were measured using Western blot analysis; (B) effect of interference of GAS5 and HMGB1 expression on p65 nuclear translocation by immunofluorescence assay. To each group, n=6; data were exhibited as mean ± SD, and measured using two-way ANOVA; **, P<0.01, compared with the Sepsis + LV-NC group; ##, P<0.01, compared with the Sepsis + LV-GAS5-shRNA + oe-NC group.