FIGURE 3.

Xanthomonas campestris pv. campestris (Xcc) transcription activator‐like effectors (TALEs) activate the expression of the ERF121 gene. (a) Composition and location of the effector‐binding element (EBE) in the ERF121 promoter. The vertical dashes indicate matches between the EBE and repeat variable diresidues. The putative start codon is highlighted in red. (b) Expression level of ERF121 in the leaf tissues of Brassica oleracea line TO1000DH3 on syringe infiltration with TALE‐bearing (XY1‐1, XY2‐1) or TALE‐less (Tir2, Ram2‐2, Bun‐1) Xcc strains or mock at 6, 12, 24, and 48 hr postinoculation (hpi). Expression in the sample after mock inoculation at 48 hpi is considered a single expression unit. The bars show relative expression fold changes ± SEM. Three reference genes (Actin‐2, GAPDH, EF1α) were used to calculate relative expression