FIGURE 6.

An 86 amino acid fragment of FoEG1 was sufficient to induce cell death in Nicotiana benthamiana. (a) Schematic presentation of FoEG1 mutants (replaced C³³, C⁶², and C²³⁷ with Ala). (b) Cell death‐inducing activity of the indicated mutant proteins was determined 5 days after agroinfiltration by transient expression in 4‐week‐old N. benthamiana leaves, and INF1 and green fluorescent protein (GFP) were used as positive and negative controls, respectively. (c) Immunoblot analysis of proteins from N. benthamiana leaves transiently expressing the indicated mutant proteins. (d) Various truncated mutants of FoEG1 were constructed and transiently expressed by agroinfiltration in 4‐week‐old N. benthamiana leaves. Photographs were taken 5 days after agroinfiltration. Transient expression of FoEG1 mutants that could not cause cell death was confirmed by immunoblot analysis, with anti‐FLAG antibody labelled “F” and Ponceau S‐stained RuBisCO protein labelled “R”