FIGURE 6.

Phytophthora infestans genes encoding putative secreted metalloproteases (MPs) and their capacity to promote or inhibit virulence. MPs are shown with a predicted signal peptide (⊳), signal anchor (◻), or mitochondrial transit peptide (❍). Numbers in grey squares refer to expression clusters (Figure 5). MPs marked with an asterisk (*) have accessory domains. Bars on the right show lesion sizes on Nicotiana benthamiana leaves transiently expressing MP genes at 5 days postinoculation. Inoculation with zoospores of P. infestans strain 14‐3‐GFP was performed one day after agroinfiltration. Bar colours indicate significantly smaller (red) or larger (green) lesions or no effect (grey) when compared to the empty vector control (EV, black; analysis of variance, p < .05). Three MPs (n.d.) were not tested in planta. Bars represent average lesion sizes from three biological repeats (n = 20). Error bars indicate SD