Figure 7. Efficacy of suicide gene therapy in glioblastoma using PEG-PBAE/pHSV-tk NP and ganciclovir.

(A) Confocal images and flow cytometry scatter plots demonstrating the efficient transfer of calcein-AM dye from pre-incubated GBM1A WT cells into co-cultured GBM1A-mCherry cells through active gap junctions (scale bar = 50 μm). (B) Experimental schedule for survival study in GBM1A-bearing orthotopic glioblastoma model in mice, and (C) Kaplan-Meier survival plot between mice treated with intratumoral injection of either PEG-PBAE/pHSV-tk NP or PEG-PBAE/pDsRed NPs in conjunction with systemic ganciclovir (n ≥ 4 per group).