Fig. 4.

OTULIN silencing inhibited the neuroprotective effect of EA in focal cerebral ischaemia/reperfusion rats. Gene interference with OTULIN expression was assessed using RT-qPCR (a, n = 3) and Western blotting (b and c, n = 3). Neurobehavioural function was evaluated using the mNSS score (d, n = 10), inclined board test (e, n = 10), and rotarod test (f, n = 10). g The graph shows an image of TTC (white: infarct area; red: noninfarct area). (h, n = 5) The panel presents the quantitative analysis of the cerebral infarct volume. ***P < 0.001 and **P < 0.01 versus the Sham group; &&&P < 0.001, &&P < 0.01, and &P < 0.05 versus the tMCAO group; $$$P < 0.001 and $$P < 0.01 versus the tMCAO + EA group; ###P < 0.001 and ##P < 0.01 versus the tMCAO + EA + LV-Scramble group