Fig. 3.

Recruitment of hMSCs towards primary tumor was CXCR4-dependent. CM-DiI-labeled hMSCs pre-treated with or without 10μg/ml AMD3100, the specific CXCR4 antagonist, were injected into mice (n = 4 for PBS control group and AMD3100 pre-treated hMSCs group) with neuroblastoma via tail vein at Day 48 after orthotopic tumor engraftment. By detecting the fluorescence signal by CRI in vivo imaging system, hMSCs were observed to be preferentially attracted by primary tumor which was consistently, (although not statistically significant P = 0.08, n = 3, representative results of 2 mice shown) inhibited by AMD3100. Data was analyzed by unpaired one-tailed t-test. The data graphs were conducted by GraphPad Prism 5