Fig. 5.

Recruitment of hMSCs by metastatic loci could not be completely inhibited by CXCR4 specific antagonist AMD3100. We traced the hMSCs trafficking in vivo by detecting the fluorescence signal of CM-DiI pre-labeled hMSCs in freshly harvested livers and lungs in Day 56 after orthotopic tumor engraftment. Fluorescence image quantified data was analyzed by un-paired one-tailed t-test. 10μg/ml AMD3100 pre-treatment could not significantly inhibit the recruitment of hMSCs towards the liver with metastatic disease (P = 0.4465, n = 3, representative results of 2 mice shown) (a), but could significantly block the recruitment towards the lung invaded with tumor cells, ^*indicates P = 0.04548 < 0.05 (n = 3, representative results of 2 mice shown) (b). The statistical analysis and data graphs were conducted by GraphPad Prism 5