Fig. 7.

NLRP7 facilitates transactivation of CCL2 via NF-κB. a Phosphorylation of p65 was increased in NLRP7-overexpressing cells, whereas the protein levels did not change. b Immunofluorescence analysis demonstrated that NLRP7 overexpression increased p65 NF-κB accumulation in the nucleus. Scale bars, 10 μm. c NF-κB-specific inhibitor CAPE suppressed the NLRP7-induced expression of CCL2. d Luciferase reporter gene analysis showed that ectopic expression of NLRP7 induced, whereas CAPE suppressed CCL2 transcription. e A schematic diagram displays high level of NLRP7 protein deubiquitination by USP10 promoting tumor-associated macrophage polarization via NF-κB-CCL2 signaling in colorectal cancer. ***P < 0.001, **P < 0.01, and *P < 0.05. NS: no significance