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. 2021 Apr 10;40:127. doi: 10.1186/s13046-021-01912-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — ASS1 acquires tumor suppressor activity independently of arginine metabolism and the p53 pathway a The NO production in ASS1-upregulation or downregulation in HCC cells. b ASS1 and CHOP expression levels and c cell survival analysis in pCMV3 or ASS1-treansfected Huh7 cells with the NO scavenger treatment; cupral (left panel) and cPTIO (right panel). d Phosphorylation of H2AX (γ-H2AX) and p53 pathway related proteins expression level in pCMV3- or ASS-flag transfected p53 wild type cells (Fa2N-4 and HepG2 cells) and e Colony formation assay in pCMV3- or ASS1-flag transfected p53-wild type cells. f Expression of γ-H2AX p53 related proteins in p53-null cell (Hep3B) and p53-mutant cells (Huh7 and SNU475 cells). g Colony formation assay in pCMV3- or ASS1-flag transfected p53-null and p53-mutant cells. ^*P < 0.05, ^**P < 0.01 compared to control group. At least triplicate analysis in each set of experiment. ns; not significant, NO; nictic oxide