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. 2020 Aug 25;48(3):851–858. doi: 10.1007/s00259-020-04984-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Algorithmic-defined VOIs for all hotspots within the scanned area for both [^99mTc]Tc-tilmanocept and [^99mTc]Tc-nanocolloid. Summed intensity projections of SPECT reconstructions of the same patient, injected with either [^99mTc]Tc-tilmanocept (left) or [^99mTc]Tc-nanocolloid (right). Injection site: Red hotspot. Reference source: Blue hotspot. ‘Hot’ lymph nodes: Green hotspots with coloured VOIs. b Verification of VOIs containing ‘hot’ lymph nodes using 3D segmentation software (ITK-SNAP). Sentinel lymph nodes: Red and green VOI. Higher echelon nodes: blue, yellow, turquoise, and purple VOI