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. 2021 Mar 30;13(7):1577. doi: 10.3390/cancers13071577

Figure 4.

Figure 4

Evaluation of NK cell persistence in mice. NK cells were isolated from buffy coats of three donors, activated using Protocol B (memory-like NK cells) or, alternatively, with rhIL-2 Overnight (control NK). At the end of the culture, the cells were cryopreserved and after thawing their potency was confirmed against THP-1 cell lines before transferring them into mice. (A) Cytotoxic activity of memory-like NK cells derived from buffy coats of three healthy donors (BC-A, BC-B and BC-C) and from a mix of the three buffy coats (Mix A + B + C). The dashed line represents the cytotoxic activity of NK cells from a mixture of buffy coats activated ON with rhIL-2 (control NK). (B) Kinetics of recovery of memory-like and control NK cells after transfer into mice. The recovery of memory-like NK cells was higher than that of control NK starting from day +3 to day +19 after in vivo transfer (* p < 0.01).