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. 2021 Mar 24;26(7):1825. doi: 10.3390/molecules26071825

Figure 2.

Figure 2

Nano-LC chromatograms of the separation of the four cannabinoids on different stationary phases: capillary columns (100 µm × 25 cm) packed with (A) Cogent Bidentate C18 dp 4.2 µm, (B) Gemini C18 dp 5 µm, (C) ChromSpher C18 dp 3 µm; mobile phase, acetonitrile/H2O/formic acid, 80/19/1% (v/v/v); UV detection, 214 nm; standard mixture concentration, cannabigerol (CBG) and cannabidiol (CBD) at the concentration of 15 µg/mL, cannabichromene (CBC) and Δ9-tetrahydrocannabinol (Δ9-THC) at a concentration of 30 µg/mL; injection time, 15 s; flow rate, 550 nL/min. (1) CBG, (2) CBD, (3) Δ9-THC, (4) CBC.