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. 2021 Mar 24;22(7):3307. doi: 10.3390/ijms22073307

Figure 5.

Figure 5

MiR-21 mediates fibrogenic activation in human cardiac fibroblasts. (A) Schematic representation of miR-21 overexpression by nucleofection of human cardiac fibroblasts with the expression vector and miR-21 inhibition by LNA anti-miR-21. (B) MiR-21, COL1A1, COL1A2, SPRY1, CADM1, and STAT3 expression levels 48h after nucleofection with miR-21 expression vector (pDNAmiR-21), measured by RT-qPCR (Fold Change to non-stimulated control). miRNA was normalized to miR-423-3p, and mRNAs were normalized to HPRT/GAPDH. (C) MiR-21, COL1A1, COL1A2, SPRY1, CADM1, and STAT3 expression levels after TGFβ1 stimulation or treated with LNA-anti-miR-21 for 24 h, measured by RT-qPCR (Fold Change to non-stimulated control). miRNA was normalized to miR-423-3p, and mRNAs were normalized to HPRT/GAPDH. Results are expressed as mean ± SEM of three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.000.1 Student’s t-test.