Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Apr 1;22(7):3662. doi: 10.3390/ijms22073662

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

The formation of 48S complexes on mRNAs with the beta-globin leader. Left panel, capped unmodified mRNA (Cap-5′UTR_b-gl); middle panel, capped m⁶A-modified mRNA (Cap-5′UTR_b-gl (m⁶A)); right panel, uncapped m⁶A-modified mRNA (5′UTR_b-gl (m⁶A)). Capillary electrophoresis electropherograms of mRNA toeprints are presented. The peak on the 5′ end corresponds to the full-length reverse-transcription product: the peaks on AUG—to 48S complexes. The calculated specific fluorescence intensity is indicated above each 48S peak (%). Quantification is relative (the proportion between peaks) for each electropherogram.