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The efficiency of the formation of 48S complexes on mRNAs with the beta-globin leader. Red column, capped unmodified mRNA (Cap-5′UTRb-gl); green column, capped m6A-modified mRNA (Cap-5′UTRb-gl (m6A)); blue column, uncapped m6A-modified mRNA (5′UTRb-gl (m6A)). The histogram is based on the specific fluorescence intensities corresponding to 48S peaks of the toeprinting reaction (Figure 2). Y-axis, fluorescence percentage at the AUG codon.
