Figure 4.

The translation of methylated mRNA shows lower sensitivity to the cap-analog-induced inhibition of cap-dependent translation. C + A + Firefly luciferase reporter mRNA carrying the beta-globin 5′ UTR (0.15 nmol) was translated in HEK293T cell extract with or without a cap analog (0.1 mM). The reaction mixture was incubated for 60 min at 30 °C before the determination of Firefly luciferase activity, which was measured as 100% with no cap analog added. The results of three independent experiments are presented. Bars are 2 standard deviations. A two-tailed Student’s t-test was used to estimate the statistical significance; *** p < 0.001. The absolute values of luciferase activity are plotted in Supplementary Figure S2.